Journal: Frontiers in Immunology

Article Title: Resistance training alleviates muscle atrophy and muscle dysfunction by reducing inflammation and regulating compromised autophagy in aged skeletal muscle

doi: 10.3389/fimmu.2025.1597222

Figure Lengend Snippet: Resistance training regulated mTORC1-HIF-1α pathway and alleviated chronic inflammation. (A) Representative Western blot images of mTOR, Raptor, HIF-1α, p70S6K, p-AKT (Ser473), AKT, IL-1β, and the internal control GAPDH in the quadriceps muscle. (B–H) Protein expression levels of p-AKT (Ser473), p-AKT (Ser473)/AKT, mTOR, Raptor, HIF-1α, p70S6K, and IL-1β. All data are presented as means ± SEM (two-way ANOVA with Tukey post hoc test), n = 6 per group; *p < 0.05, **p < 0.01.

Article Snippet: Membranes were blocked at room temperature for 1 h with 5% non-fat dry milk (Bio-Rad, 1706404) in Tris-buffered saline with Tween-20 (TBST) and incubated overnight at 4°C with primary antibodies: p-AKT (Ser473) (Cell Signaling Technology, #86758, 1:1000), AKT (Cell Signaling Technology, #9272, 1:1000), p-AMPKα (Thr172) (Cell Signaling Technology, #2535, 1:1000), AMPKα (Cell Signaling Technology, #2532, 1:1000), mTOR (Cell Signaling Technology, #2983, 1:1000), Raptor (Cell Signaling Technology, #2280, 1:1000), p70S6K (Cell Signaling Technology, #9202, 1:1000), IL-1β (Proteintech, 16806-1-AP, 1:2000), HIF-1α (Cell Signaling Technology, #14179, 1:1000), Beclin1 (Proteintech, 11306-1-AP, 1:1000), TFEB (Proteintech, 13372-1-AP, 1:1000), p62 (Proteintech, 18420-1-AP, 1:1000), LC3 (Cell Signaling Technology, #2775, 1:2000), and GAPDH (Proteintech, 60004-1-Ig).

Techniques: Western Blot, Control, Expressing

Journal: Molecular medicine reports

Article Title: Chinese herb extract improves liver steatosis by promoting the expression of high molecular weight adiponectin in NAFLD rats.

doi: 10.3892/mmr.2017.7284

Figure Lengend Snippet: Figure 5. Expression of proteins in adipose tissue analyzed using western blot analysis. Results of the western blot analysis are showed in graph. (A and B) Optical density value ratios of target protein to GAPDH are showed in bar graphs. **P<0.01, T group vs. M group; §§P<0.01, T group vs. CK group; #P<0.05, M group vs. CK group; ##P<0.01, M group vs. CK group. CK, normal control group; M, model control group; T, treatment group; HMW APN, high molecular weight adiponectin; FOXO1, forkhead box O protein 1; DsbA‑L, disulfide‑bond A oxidoreductase‑like protein; p‑, phosphorylated.

Article Snippet: The primary antibodies were as follows: GADPH (cat. no. AP0063; 1:5,000; Bioworld Technologies, Inc., St. Louis Park, MN, USA), Akt (cat. no. ab9150; 1:2,000; Abcam, Cambridge, UK), phosphorylated (p)-Akt (cat. no. 2965; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA USA), FOXO1 (cat. no. 2880; 1:10,00; Cell Signaling Technology, Inc.), p-FOXO1 (cat. no. 9461; 1:1,000; Cell Signaling Technology, Inc.), DsbA-L (cat. no. ab92819; 1:2,000; Abcam, Cambridge, UK) and APN (cat. no. bs-0471R; 1:300; Bioss, Beijing, China).

Techniques: Expressing, Western Blot, Control, High Molecular Weight